Home    Primer Tools    ASPE Tools    Downloads    Documentation    About Us		Primer Tools Input: Sequence Data File:     (format) Note: Maximum of 100 sequences :: 75 nucleotides Parameters: Monovalent Salt (Molar)   (Explain this)   Total Strand Concentration (µM)   (Explain this) Temperature (ΔG calculations)(°C)   (Explain this) Total Score Required  -20 -19 -18 -17 -16 -15 -14 -13 -12 -11 -10 -9 -8 -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 (Explain this) Calculate Primer Statistics Results Calculate Auto Dimer Results Calculate Hairpin Results
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