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105.8 - DNA Profiling and Nucleic Acid Materials (solid forms)

Standard Reference Material (SRM) 2372a is intended primarily for use in the value assignment of human genomic deoxyribonucleic acid (DNA) forensic quantitation materials. SRM 2372a consists of three well-characterized human genomic DNA materials in pH 8.0 aqueous buffer. The components are derived from human buffy coat samples and labeled A, B, and C. Component A consists of genomic DNA from a single male donor. Component B consists of genomic DNA from a single female donor. Component C consists of a gravimetric mixture of genomic DNA (1 part male donor to 3 parts female donor). SRM 2372a is certified for copy number and DNA concentration (ng/µL).  A unit of the SRM consists of one sterile 0.5 mL vial of each component, each vial containing approximately 55 μL of DNA solution. Each of these vials is labeled and is sealed with a color -coded screw cap.

SRM 2374 is intended for use as a template for ribonucleic acid (RNA) control synthesis using in vitro transcription (IVT).  These RNA controls are designed to be used as external, or “spike-in”, controls to support confidence in gene expression assays by providing quantitative assessment of the technical performance of a gene expression measurement.  A unit of the SRM contains 96 different 0.5 mL polypropylene tubes, with approximately 10 µg of dehydrated plasmid deoxyribonucleic acid (DNA) in each tube. Depending on the strand transcribed, the controls will mimic either “sense” or “anti-sense” eukaryotic messenger RNA (mRNA).  


SRM 2391d is intended for use in the standardization and calibration of forensic and paternity quality assurance procedures and instructional law enforcement or non-clinical research purposes.  SRM 2391d includes short tandem repeat (STR), single nucleotide polymorphism (SNP), and whole mitochondrial genome DNA (mtDNA) sequence information for all samples in the SRM. The STR data includes the Federal Bureau of Investigation's (FBI's) CODIS (Combined DNA Index System) core STR loci and additional forensically relevant loci. Certified Values for a total of 35 autosomal STR loci plus Amelogenin, 28 Y-STR loci, and seven X-STR loci, as well as Information Values for 13 autosomal STR loci, three Y-STR loci, five X-STR loci, 30 Insertion and Deletion (Indel) loci, 20 Insertion and Null allele (INNUL) loci, 101 autosomal identity SNP loci, 34 Y-SNP loci, 188 autosomal ancestry and phenotype SNP loci, and mtDNA whole genome sequences are included in this issue. The new standard includes well-characterized DNA in two forms: genomic DNA and DNA contained within cells spotted onto FTA filter papers. SRM 2391d consists of five components packaged in one box and stored at 2 oC to 4 oC (do not freeze).

 

SRM 2392 is intended to provide quality control when performing the polymerase chain reaction (PCR) and sequencing of human mitochondrial DNA (mtDNA) for forensic identifications, medical diagnosis, or mutation detection. It may also be used as a control when amplifying (PCR) and sequencing any DNA.  SRM 2392 Mitochondrial DNA Sequencing contains DNA extracted from two cell lines plus cloned DNA from a region that is difficult to sequence. The certificate accompanying the SRM details the base pair sequences of the DNA, and the sequences of 58 unique primer sets which permit the amplification and sequencing of any specific area or the entire human mitochondrial DNA (strand).  SRM 2392 consists of three frozen components packaged in one box.  For further information see: SP260-155

SRM 2392-I Mitochondrial DNA Sequencing compliments and adds another DNA template to SRM 2392 for the amplification and sequencing of human mtDNA. The selection of the HL-60 cell culture line for this additional DNA template was based on a suggestion from the Federal Bureau of Investigation (FBI) that this DNA would be particularly useful to the forensic community.  For further information see: SP260-155

RMs 8366, 8375, 8391, 8392, 8393, and 8398 are intended for assessing performance of human genome sequencing, including whole genome sequencing, whole exome sequencing, and more targeted sequencing such as gene panels. Specifically, the material can be used to obtain estimates of true positives, false positives, true negatives, and false negatives for variant calls.

PLEASE NOTE: The tables are presented to facilitate comparisons among a family of materials to help customers select the best SRM for their needs. For specific values and uncertainties, the certificate is the only official source.


- Certified values are normal font
- Reference values are italicized
- Values in parentheses are for information only


Detail - Detail    Certificate - Certificate    Report of Investigation - Report of Investigation    MSDS - MSDS    Data Files - Data Files    Questionaire - Questionnaire


   
  SRM Description Unit of Issue    
Detail Certificate MSDS 2365
Now Selling
BK Virus DNA Quantitative Standard 1 vial x 110 µL  
Detail Certificate MSDS 2366a
Now Selling
Cytomegalovirus DNA (TowneΔ147 BAC) for DNA Measurements 1 vial x 150 µL  
Detail Certificate MSDS 2372a
Now Selling
Human DNA Quantitation Standard 3 vials x 55 µL  
Detail Certificate MSDS 2373
Now Selling
Genomic DNA Standards for HER2 Measurements 5 vials, 1 each level  
Detail Certificate MSDS Data Files 2374
Now Selling
DNA Sequence Library for External RNA Controls 96 tubes  
Detail Certificate MSDS Data Files 2391d
Now Selling
PCR-Based DNA Profiling Standard 5 vials  
Detail Certificate MSDS 2392
Now Selling
Mitochondrial DNA Sequencing (Human)
set (3)  
Detail Certificate MSDS 2392-I
Now Selling
Mitochondrial DNA Sequencing (Human HL-60 DNA)
each  
Detail Certificate MSDS 2393
Now Selling
CAG Repeat Length Mutation in Huntington's Disease set (6)  
Detail Certificate MSDS 2396
Now Selling
Oxidative DNA Damage Mass Spectrometry Standards set (10)  
Detail Report of Investigation MSDS 8366
Now Selling
EGFR and MET Gene Copy Number Standards for Cancer Measurements
6 vials, 100 µL each  
Detail Report of Investigation MSDS 8375
Now Selling
Microbial Genomic DNA Standards for Sequencing Performance Assessment
(MG-001, MG-002, MG-003, MG-004)
4 vials, 1 each  
Detail Report of Investigation MSDS 8391
Now Selling
Human DNA for Whole-Genome Variant Assessment (Son of Eastern European Ashkenazi Jewish Ancestry) (HG-002) 1 vial  
Detail Report of Investigation MSDS 8391(QTY10)
Now Selling
Human DNA for Whole-Genome Variant Assessment (Son of Eastern European Ashkenazi Jewish Ancestry) (HG-002) 10 vials of RM 8391  
Detail Report of Investigation MSDS 8392
Now Selling
Human DNA for Whole-Genome Variant Assessment (Family Trio of Eastern Europe Ashkenazi Jewish Ancestry) (HG-002, HG-003, HG-004) 3 vials, 1 each  
Detail Report of Investigation MSDS 8393
Now Selling
Human DNA for Whole-Genome Variant Assessment (Son of Chinese Ancestry) (HG-005) 1 vial  
Detail Report of Investigation MSDS 8393(QTY10)
Now Selling
Human DNA for Whole-Genome Variant Assessment (Son of Chinese Ancestry) (HG-005) 10 vials of RM 8393  
Detail Report of Investigation MSDS 8398
Now Selling
Human DNA for Whole-Genome Variant Assessment (Daughter of Utah/European Ancestry)(HG-001) 1 vial  
Detail Report of Investigation MSDS 8398(QTY10)
Now Selling
Human DNA for Whole-Genome Variant Assessment (Daughter of Utah/European Ancestry) (HG-001) 10 vials of RM 8398  
Detail Report of Investigation MSDS 8671
Now Selling
NISTmAb, Humanized IgG1κ Monoclonal Antibody 1 vial x 800 uL