Data and Information Files

SRM 2374 - DNA Sequence Library for External RNA Controls

The certified sequence data file is: SRM2374_Sequence_v1.FASTA.

The file containing the certified confidence estimates for every base of sequence is: SRM2374_Quality_v1.FASTA.

Secondary files are available for accurate and convenient use in sequence mapping applications. These include a simplified representation of the sequence data with IUPAC ambiguity codes resolved using a best likelihood estimate of the nucleotide (with and without a polyA tail), a nominal vector sequence, and "Gene Transfer Format" (GTF) files.

- SRM2374_ambiguities_resolved_v2.FASTA is the sequence datafile with IUPAC ambiguity codes resolved;

- SRM2374_ambiguities_resolved_v1.GTF describes the structures of the RNA controls as if they were genes;

- SRM2374_ambiguities_resolved_NoPolyA_v2.FASTA is the sequence reference file with the polyA tail excluded;

- SRM2374_ambiguities_resolved_NoPolyA_v1.GTFis a GTF with the polyA tail excluded;

- SRM2374_PlasmidVector_by_ERCCNumber_v1.FASTA is the sequence reference file of the plasmid vector.

- SRM2374 _putative_T7_products_NoPolyA_v2.FASTA is the putative sequence generated by in vitro transcription with T7 RNA polymerase and conversion to cDNA, with the polyA tail excluded.

These files contain certified and information data to support the use of SRM 2374. The files are text-based formats for use with programs that use data in FASTA and GTF formats.

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Last updated: 5/14/2019